{"id":13,"date":"2023-11-02T17:04:52","date_gmt":"2023-11-02T17:04:52","guid":{"rendered":"https:\/\/www-cxcp1.tifrh.res.in\/~kanchan\/?page_id=13"},"modified":"2024-01-20T08:04:24","modified_gmt":"2024-01-20T08:04:24","slug":"dr-kanchan-garai","status":"publish","type":"page","link":"https:\/\/www.tifrh.res.in\/~kanchan\/","title":{"rendered":"Single molecule biophysics Lab"},"content":{"rendered":"

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Kanchan Garai<\/h3>\n<\/div>\n<\/div>\n

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Study of Protein Amyloids and Liquid-liquid Phase Separation (LLPS) using Single Molecule Techniques:<\/strong>\u00a0Aggregation of amyloidogenic proteins are associated in the pathology of multiple human diseases, such as Alzheimer\u2019s and Parkinson\u2019s disease and Type 2 diabetes. The main goal of our research to investigate the molecular mechanisms of its aggregation. Endogenous proteins such as chaperones and apolipoprotein E are known the influence the progression of the above-mentioned diseases. We are using an array of biophysical techniques to investigate the role of these proteins on protein aggregation.<\/p>\n

Amyloid beta peptide (A\u03b2):<\/strong>\u00a0Aggregation and deposition of A\u03b2 in the brain is hallmark of Alzheimer\u2019s disease.<\/p>\n

Alpha-synuclein:<\/strong>\u00a0Primary component of the Lewy bodies and Lewy neurites in Parkinson disease and other synucleinopathies.<\/p>\n

Tau protein:<\/strong>\u00a0Tau is the major microtubule associated protein (MAP) in neurons. Aberrant aggregation of tau is involved in the pathology of Alzheimer\u2019s disease and other tauopathies.<\/p>\n

Islet amyloid polypeptide (IAPP) or Amylin:<\/strong>\u00a0A 37 -residue peptide hormone, which plays important roles in glycaemic regulation. Aggregation of IAPP inside the pancreatic islets is involved in the pathology of Type 2 diabetes mellitus (T2DM).<\/p>\n

LLPS:<\/strong> Many intrinsically disordered proteins (IDPs) undergo LLPS separating into protein dilute and protein rich phases. These LLPS subsequently mature to gel-like solids or to amyloid fibrils. We study the physicochemical properties of the LLPS of several IDPS including amyloid, alpha-synuclein and tau.<\/p>\n

Single Molecule Techniques:<\/strong> We build and use various single molecule spectroscopy and imaging techniques to study protein amyloids and LLPS droplets. The techniques built in the lab are Fluorescence Correlation Spectroscopy (FCS), Cuvette-FCS, single molecule FRET (smFRET), Two-Color Coincidence Detection (TCCD), Total Internal Reflection Fluorescence Microscopy (TIRFM), Superresolution Optical Microscopy etc.<\/p>\n

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Kanchan Garai Study of Protein Amyloids and Liquid-liquid Phase Separation (LLPS) using Single Molecule Techniques:\u00a0Aggregation of amyloidogenic proteins are associated in the pathology of multiple human diseases, such as Alzheimer\u2019s and Parkinson\u2019s disease and Type 2 diabetes. The main goal of our research to investigate the molecular mechanisms of its aggregation. Endogenous proteins such as […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-13","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.tifrh.res.in\/~kanchan\/wp-json\/wp\/v2\/pages\/13","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.tifrh.res.in\/~kanchan\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.tifrh.res.in\/~kanchan\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.tifrh.res.in\/~kanchan\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.tifrh.res.in\/~kanchan\/wp-json\/wp\/v2\/comments?post=13"}],"version-history":[{"count":7,"href":"https:\/\/www.tifrh.res.in\/~kanchan\/wp-json\/wp\/v2\/pages\/13\/revisions"}],"predecessor-version":[{"id":79,"href":"https:\/\/www.tifrh.res.in\/~kanchan\/wp-json\/wp\/v2\/pages\/13\/revisions\/79"}],"wp:attachment":[{"href":"https:\/\/www.tifrh.res.in\/~kanchan\/wp-json\/wp\/v2\/media?parent=13"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}