{"id":34,"date":"2023-11-04T20:02:52","date_gmt":"2023-11-04T20:02:52","guid":{"rendered":"https:\/\/www-cxcp1.tifrh.res.in\/~vipin\/?page_id=34"},"modified":"2024-08-06T16:19:38","modified_gmt":"2024-08-06T16:19:38","slug":"protein-structures-at-fast-mas","status":"publish","type":"page","link":"https:\/\/www.tifrh.res.in\/~vipin\/protein-structures-at-fast-mas\/","title":{"rendered":"Protein structures at fast MAS"},"content":{"rendered":"

[et_pb_section fb_built=”1″ _builder_version=”4.23″ _module_preset=”default” custom_padding=”5px|||||” global_colors_info=”{}”][et_pb_row _builder_version=”4.23″ _module_preset=”default” border_width_bottom=”1px” border_color_bottom=”#e2e2e2″ global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.23″ _module_preset=”default” global_colors_info=”{}”][et_pb_post_title meta=”off” featured_image=”off” _builder_version=”4.23″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_post_title][\/et_pb_column][\/et_pb_row][et_pb_row use_custom_gutter=”on” gutter_width=”2″ _builder_version=”4.23″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.23″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.23″ _module_preset=”default” custom_padding=”0px|||||” global_colors_info=”{}”]<\/p>\n

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R<\/span>apid data acquisition for de novo\u00a0<\/i>structure determination of proteins<\/span><\/h4>\n

<\/span><\/p>\n

We combine well-known techniques such as SIM-CP, co-evolution, bidirectional cross-polarization, orphan pathways and multiplexing, pioneered by several other groups in the field, to design proton-detected residue linking experiment.\u00a0<\/span><\/span>This sequence allows simultaneous acquisition of one new, CA(CON)CAHA<\/sup>and five previously known experiments NCAHA<\/sup>, N(CO)CAHA<\/sup>, CANH, CA(CO)NH and N(CACO)NH.<\/span>48<\/span><\/sup>For the microcrystalline ubiquitin sample, all these spectra <\/span>could be recorded in about 1.7 days compared to about 5-7 days of measurement time required to independently acquire all the spectra. \u00a0These six spectra allow independent forward and backward backbone walks using the resolved 2D NHN<\/sup>and CAHA<\/sup>planes. Rapid data acquisition combined with automated assignment makes this a compelling approach for de novo <\/i>structural characterization of protein using nanomolar sample amounts.\u00a0<\/span><\/p>\n<\/div>\n

[\/et_pb_text][et_pb_image src=”https:\/\/www.tifrh.res.in\/~vipin\/wp-content\/uploads\/2024\/08\/Screenshot-from-2024-08-06-21-51-13.png” title_text=”Screenshot from 2024-08-06 21-51-13″ _builder_version=”4.23″ _module_preset=”default” hover_enabled=”0″ global_colors_info=”{}” sticky_enabled=”0″][\/et_pb_image][et_pb_text _builder_version=”4.23″ _module_preset=”default” global_colors_info=”{}”]<\/p>\n

Figure: Two Independent backbone walks using two-dimensional HN and HaCa planes<\/p>\n

This project is a collaborative effort with a colleague, Dr. Kaustubh Mote, at TIFR Hyderabad.<\/em><\/p>\n

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Rapid data acquisition for de novo\u00a0structure determination of proteins We combine well-known techniques such as SIM-CP, co-evolution, bidirectional cross-polarization, orphan pathways and multiplexing, pioneered by several other groups in the field, to design proton-detected residue linking experiment.\u00a0This sequence allows simultaneous acquisition of one new, CA(CON)CAHAand five previously known experiments NCAHA, N(CO)CAHA, CANH, CA(CO)NH and N(CACO)NH.48For […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-34","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.tifrh.res.in\/~vipin\/wp-json\/wp\/v2\/pages\/34"}],"collection":[{"href":"https:\/\/www.tifrh.res.in\/~vipin\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.tifrh.res.in\/~vipin\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.tifrh.res.in\/~vipin\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.tifrh.res.in\/~vipin\/wp-json\/wp\/v2\/comments?post=34"}],"version-history":[{"count":4,"href":"https:\/\/www.tifrh.res.in\/~vipin\/wp-json\/wp\/v2\/pages\/34\/revisions"}],"predecessor-version":[{"id":131,"href":"https:\/\/www.tifrh.res.in\/~vipin\/wp-json\/wp\/v2\/pages\/34\/revisions\/131"}],"wp:attachment":[{"href":"https:\/\/www.tifrh.res.in\/~vipin\/wp-json\/wp\/v2\/media?parent=34"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}